Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes.
When an insect molts, old cuticle on the outside of the integument is shed by apolysis and a new cuticle is formed under the old one.This process is completed by the epidermal cells which are controlled by 20-hydroxyecdysone (20E) and juvenile hormone.To understand the molecular mechanisms of integument remolding and hormonal regulation on the gene